Dynamic Light Scattering (DLS)

Dynamic Light Scattering (DLS) is a powerful, non-destructive technique that illuminates the behavior of proteins, macromolecules, and nanoparticles in solution. DLS passes a laser beam through a sample and the biomolecules in the sample scatter the laser light. The resulting scattering pattern reveals essential insights into the molecules’ size, stability, homogeneity, and their tendency to aggregate.

We use he Prometheus Panta (NanoTemper) for highly precise DLS analysis. The system offers a remarkable sensitivity, enabling the analysis of challenging samples at low concentrations.

Deduced Parameters

rH - Hydrodynamic radius; the (average) size of the particles in solution

PDI - Polydispersity index; distribution of particle size populations

Tsize - Temperature at which average particle size begins to increase

Tscattering Temperature at which particles begin to form scattering aggregates, from change of scattering intensity

Liquid-Liquid-Phase-Separation (LLPS) analysis

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